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Effects of HIF-1 alpha on diabetic retinopathy angiogenesis and VEGF expression 期刊论文

编号：

5ecdd6a1-ae6a-41fa-9c79-ee8bb38f4399
作者：

Zhang, D.(张丹)#*^[1]Lv, FL(吕富丽)^[1]Wang, GH(王广慧)^[1]
语种：

英文
期刊：

EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES ISSN：1128-3602 2018 年 22 卷 16 期 (5071 - 5076) ; AUG
收录：
关键词：

Hypoxia inducing factor Antisense oligonucleotide Vascular endothelial growth factor Angiogenesis Diabetic retinopathy
摘要：

OBJECTIVE: To investigate the effect of hypoxia inducing factor (HIF)-1 alpha on the expression of vascular endothelial growth factor (VEGF) and angiogenesis in diabetic retinopathy.
PATIENTS AND METHODS: 8-week healthy SD rats were used for the experiments. Under systemic anesthesia condition, control rats received a saline injection into the left ocular body (control A group), and 2 mu l antisense oligonucleotides (ASODN) (10 mu mol/L) into right eye (control B group). Model rats received a saline injection into the left eye (model A group), and 2 mu l ASODN (10 mu mol/L) into the right eye (model B group). Rats received an intraocular injection of HIF-1 alpha ASODN for 2, 4, and 6 weeks (A1, A2. A3, B1, B2, B3, respectively). Retinal vessel development was observed by ADP staining. Vascular endothelial cells penetrating retinal inner membrane were counted. Immunohistochemistry was used to detect expressions of VEGF and HIF-1 alpha proteins in the retina.
RESULTS: Prominent angiogenesis and hyperplasia were found in model A group. Relatively fewer newly formed vessels were shown in model group B. However, no significant change of retinal vascular morphology was presented in control group. Of note, the vascular endothelial cell counts, VEGF and HIF-1 alpha contents were significantly increased in model group (p < 0.05). After treatment with HIF-1 alpha ASODN, lower endothelial cell counts was found in model B group (p < 0.05 comparing to model A). VEGF expression in model B group was significantly decreased, among which, model B3 was observed with lower cell counts than model B1 or B2 (p < 0.05 comparing to model A). Injection of HIF-1 alpha ASODN significantly suppressed HIF-1 alpha level in model B in a time-dependent manner.
CONCLUSIONS: Retinal angiogenesis is closely related with increasing level of HIF-1 alpha. Inhibition of HIF-1 alpha suppressed VEGF expression and deterred angiogenesis in a time-dependent manner. This provided novel insights for treating diabetic retinopathy.
推荐引用方式
GB/T 7714：

Zhang D.,Lv F-L,Wang G-H, et al. Effects of HIF-1 alpha on diabetic retinopathy angiogenesis and VEGF expression [J].EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES,2018,22(16):5071-5076.
APA：

Zhang D.,Lv F-L,Wang G-H.(2018).Effects of HIF-1 alpha on diabetic retinopathy angiogenesis and VEGF expression .EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES,22(16):5071-5076.
MLA：

Zhang D., et al. "Effects of HIF-1 alpha on diabetic retinopathy angiogenesis and VEGF expression" .EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES 22,16(2018):5071-5076.
入库时间：

2/23/2021 9:01:28 AM
更新时间：

2/23/2021 9:01:28 AM
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