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cofilin signaling axis-regulated F-actin reassembly 期刊论文

编号：

6698D6B1AB76912AEEDFBDFCAB018DD5
作者：

Chen, Xinlan#^[1,2]Li, Zhongchen(李中辰)^[3]Men, Junqi^[1,2];Shao, Hui^[1,2];Bai, Juncheng^[1,2];Guo, Yingying^[1,2];Chen, Xing^[1,2];Fan, Yubo^[1,2];Jiang, LinHua*^[4,5,6,7]Jia, Xiaoling*^[1,2]
语种：

英文
期刊：

REGENERATIVE BIOMATERIALS ISSN：2056-3418 2026 年 13 卷
收录：
关键词：

substrate stiffness Piezo1 channel microglial cells migration F-actin reassembly
摘要：

The local stiffness of brain tissues increases in pathological states, and then, drives microglial cells to migrate towards stiffer areas for their functioning, but the underlying mechanism is still unclear. Herein, we investigated the role and mechanism of the mechanosensitive Piezo1 channel in matrix stiffness regulation of microglial cell migration using BV2 microglial cell line and mouse primary microglial cells growing on soft (500 Pa) and stiff (20 kPa) polyacrylamide (PA) hydrogels. Compared with soft substrates, stiff substrates promoted cell migration, upregulated the Piezo1 expression, raised intracellular Ca2+ concentration ([Ca2+]i) and favored F-actin reassembly. Cell migration and F-actin assembly were suppressed by inhibiting the Piezo1 channel, reducing the [Ca2+]i or inhibiting PI3K/AKT. In addition, stiff substrates induced AKT activation that was reversed by blocking the Piezo1 channel or preventing an increase in [Ca2+]i. Finally, stiff substrates induced a Piezo1-, Ca2+- and AKT-dependent decrease in the phosphorylation level of cofilin, which promotes cofilin severing F-actin, increases G-actin levels and further enhances F-actin reassembly, thereby promoting microglial cell migration. Collectively, our study has revealed that stiff matrix regulates drives microglial cell migration through the Piezo1/Ca2+/AKT/cofilin signaling axis-regulated F-actin reassembly. Our findings provide new insights into the mechanisms underlying tissue stiffness regulation of microglial cells occurring in neurological disorders.
推荐引用方式
GB/T 7714：

Chen Xinlan,Li Zhongchen,Men Junqi, et al. Stiff matrix drives microglial cell migration through Piezo1/Ca2+/AKT/cofilin signaling axis-regulated F-actin reassembly [J].REGENERATIVE BIOMATERIALS,2026,13.
APA：

Chen Xinlan,Li Zhongchen,Men Junqi,Shao Hui,&Jia Xiaoling.(2026).Stiff matrix drives microglial cell migration through Piezo1/Ca2+/AKT/cofilin signaling axis-regulated F-actin reassembly .REGENERATIVE BIOMATERIALS,13.
MLA：

Chen Xinlan, et al. "Stiff matrix drives microglial cell migration through Piezo1/Ca2+/AKT/cofilin signaling axis-regulated F-actin reassembly" .REGENERATIVE BIOMATERIALS 13(2026).
入库时间：

2/22/2026 9:22:30 PM
更新时间：

2/22/2026 9:22:30 PM

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