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Traumatic Brain Injury-Mediated Neuroinflammation and Neurological Deficits are Improved by 8-Methoxypsoralen Through Modulating PPAR gamma/NF-kappa B Pathway 期刊论文

编号：

8AAA7FA3FB4A5BB495E50714674E9617
作者：

Hui, Yuzuo(惠浴祚)#^[1]Zhao, Han#^[2]Shi, Lei^[3];Zhang, Haitao(张海涛)*^[1]
语种：

英文
期刊：

NEUROCHEMICAL RESEARCH ISSN：0364-3190 2023 年 48 卷 2 期 (625 - 640) ; FEB
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关键词：

8-Methoxypsoralen Traumatic brain injury microglia inflammation PPAR gamma NF-kappa B pathway
摘要：

8-Methoxypsoralen (8-MOP) has anti-inflammatory, antioxidant and tissue-repairing abilities. Here, we probed the function and mechanism of 8-MOP in traumatic brain injury (TBI). The in-vivo TBI model was constructed in Sprague-Dawley (SD) rats using controlled cortical impact (CCI) surgery. In parallel, BV2 microglia and HT22 neurons were activated by lipopolysaccharide (LPS) to establish an in-vitro model. The modified neurological score (mNSS) and the Morris water maze experiment were employed to evaluate the rats'' neurological functions. The rats'' brain edema was assessed by the dry and wet method, and neuronal apoptosis in damaged brain tissues was monitored by terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling (TUNEL) and Nissl''s staining. Immunohistochemistry (IHC) was applied to verify Iba1-microglial activation in brain lesions of rats. The expression of inflammatory cytokines in BV2 microglia and HT22 neurons in the injured lesion of TBI rats was examined by the enzyme-linked immunosorbent assay (ELISA). The levels of iNOS, COX2, TLR4, PPAR gamma, STAT3, and NF-kappa B in brain lesions, BV2 microglia and HT22 neurons were compared by Western blot. As a result, 8-MOP administration reduced inflammation and LPS-induced neuronal damage in BV2 microglia. In vivo, 8-MOP treatment relieved neurological deficits in TBI rats, improved cognitive, learning and motor functions and mitigated brain edema and neuroinflammation induced by TBI. Furthermore, LPS or TBI activated the NF-kappa B and STAT3 pathways and repressed the PPAR gamma expression. However, 8-MOP treatment attenuated NF-kappa B and STAT3 phosphorylation and elevated PPAR gamma levels. Hence, 8-MOP exerts neuroprotective and anti-inflammatory effects in TBI rats by modulating the PPAR gamma/NF-kappa B pathway.
推荐引用方式
GB/T 7714：

Hui Yuzuo,Zhao Han,Shi Lei, et al. Traumatic Brain Injury-Mediated Neuroinflammation and Neurological Deficits are Improved by 8-Methoxypsoralen Through Modulating PPAR gamma/NF-kappa B Pathway [J].NEUROCHEMICAL RESEARCH,2023,48(2):625-640.
APA：

Hui Yuzuo,Zhao Han,Shi Lei,Zhang Haitao.(2023).Traumatic Brain Injury-Mediated Neuroinflammation and Neurological Deficits are Improved by 8-Methoxypsoralen Through Modulating PPAR gamma/NF-kappa B Pathway .NEUROCHEMICAL RESEARCH,48(2):625-640.
MLA：

Hui Yuzuo, et al. "Traumatic Brain Injury-Mediated Neuroinflammation and Neurological Deficits are Improved by 8-Methoxypsoralen Through Modulating PPAR gamma/NF-kappa B Pathway" .NEUROCHEMICAL RESEARCH 48,2(2023):625-640.
入库时间：

4/19/2023 5:38:52 PM
更新时间：

4/19/2023 5:38:52 PM
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